1 The COMRADES experiment


The COMRADES experimental protocol for the prediction of RNA structure in vivo was first published in 2018 (Ziv et al., 2019) where they predicted the structure of the Zika virus. The protocol has subsequently been use to predict the structure of SARS-CoV-2 (Ziv et al., 2020). Have a look to get an understanding of the protocol:

  • COMRADES determines in vivo RNA structures and interactions. (2018). Omer Ziv, Marta Gabryelska, Aaron Lun, Luca Gebert. Jessica Sheu-Gruttadauria and Luke Meredith, Zhong-Yu Liu, Chun Kit Kwok, Cheng-Feng Qin, Ian MacRae, Ian Goodfellow , John Marioni, Grzegorz Kudla, Eric Miska. Nature Methods. Volume 15. https://doi.org/10.1038/s41592-018-0121-0

  • The Short- and Long-Range RNA-RNA Interactome of SARS-CoV-2. (2020). Omer Ziv, Jonathan Price, Lyudmila Shalamova, Tsveta Kamenova, Ian Goodfellow, Friedemann Weber, Eric A. Miska. Molecular Cell, Volume 80 https://doi.org/10.1016/j.molcel.2020.11.004